S&uctural Studies of the Diiron Center in Methane Monooxygenase in the Presence of Substrate and the Regulatory Protein of the MM0 Enzyme System
ثبت نشده
چکیده
منابع مشابه
X-ray Absorption Spectroscopic Studies of the Dinuclear Iron Center in Methane Monooxygenase and the Sulfure and Chlorine Centers in Photographic Materials*
The Diiron Center in Methane Monooxygenase. The dinuclear iron center of the hydroxylase component of soluble methane monooxygenase (MMO) from Methylococcus capsulatus (Bath) and Methylosinus trichosporium (OB3b) has been studied by X-ray absorption spectroscopy. Analysis of the Fe K-edge EXAFS revealed that the first shell coordination of the Fe(III)Fe(III) oxidized state of the hydroxylase fr...
متن کاملDiiron Oxidation State Control of Substrate Access to the Active Site of Soluble Methane Monooxygenase Mediated by the Regulatory Component
The regulatory component (MMOB) of soluble methane monooxygenase (sMMO) has a unique N-terminal tail not found in regulatory proteins of other bacterial multicomponent monooxygenases. This N-terminal tail is indispensable for proper function, yet its solution structure and role in catalysis remain elusive. Here, by using double electron-electron resonance (DEER) spectroscopy, we show that the o...
متن کاملBioinorganic Hydrocarbon Oxidation : Mechanistic and Kinetic Studies
Chapter 1. Principles of Small Molecule Activation by Metalloproteins as Exemplified by the Soluble Methane Monooxygenase Many metalloenzymes activate small molecules in a manner that is unique to natural systems. In this perspective we discuss the soluble protein system from Methylococcus capsulatus (Bath), which uses a mixed-function hydroxylase to convert methane selectively to methanol. Thr...
متن کاملKinetic Investigation of Myeloperoxidase upon Interaction with Copper, Cadmium, and Lead Ions
Background: Myeloperoxidase (MPO), which is abundantly expressed in neutrophils, catalyzes the formation of a number of reactive oxidant species. However, evidence has emerged that MPO-derived oxidants contribute to tissue damage and initiation and propagation of inflammatory diseases, particularly, cardiovascular diseases. Therefore, studying the regulatory mechanisms of the enzyme activity is...
متن کاملFunctional and Developmental Analysis of CD4+CD25+ Regulatory T Cells under the Influence of Streptococcal M Protein in Rheumatic Heart Disease
The purpose of this study was to determine the role of streptococcal M protein in naturally-occurring CD4+CD25+ regulatory T cells (nTregs) function and development in rheumatic heart disease in Iraqi patients. Streptococcus pyogenes was isolated for subsequent M protein extraction. Also, peripheral blood nTregs and CD4+ T cells were isolated by using Magnetic Cell Separation System. Tissue cul...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
دوره شماره
صفحات -
تاریخ انتشار 2007